Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mutations

Mutations

Mutations

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...

Point and Frameshift Mutations

Point and Frameshift Mutations

Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Logistic Regression and Machine Learning Algorithms for the Risk Prediction of Perioperative Adverse Cardiovascular Events in Elderly Patients.

Aging medicine (Milton (N.S.W))·2025

Same author

The sucrose-trehalose 6-phosphate (Tre6P) nexus: specificity and mechanisms of sucrose signalling by Tre6P.

Journal of experimental botany·2014

Same author

A mediator of singlet oxygen responses in Chlamydomonas reinhardtii and Arabidopsis identified by a luciferase-based genetic screen in algal cells.

The Plant cell·2013

Same author

Inhibition of proliferation, viability, migration and invasion of gastric cancer cells by Aurora-A deletion.

Asian Pacific journal of cancer prevention : APJCP·2012

Same author

Single-stage posterior debridement and single-level instrumented fusion for spontaneous infectious spondylodiscitis of the lumbar spine.

Acta orthopaedica Belgica·2012

Same author

Cancer prevention health services research: an emerging field.

Journal of cancer education : the official journal of the American Association for Cancer Education·2012

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Identification and Classification of Position-specific GABA_A Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

Prediction of function changes associated with single-point protein mutations using support vector machines (SVMs).

Shan Gao¹, Ning Zhang, Guang You Duan

¹Key Laboratory of Bioactive Materials, Ministry of Education and College of Life Science, Nankai University, Tianjin 300071, P.R. China.

|May 23, 2009

Summary

This study introduces a novel support vector machine (SVM) method for predicting protein function changes from single-point mutations using only sequence data. The new approach offers a faster and more accurate prediction of mutation effects.

More Related Videos

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Related Experiment Videos

Last Updated: Jun 23, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Identification and Classification of Position-specific GABA_A Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

Published on: December 9, 2015

Area of Science:

Computational biology
Bioinformatics
Protein engineering

Background:

Predicting the functional impact of single amino acid substitutions is crucial in molecular biology.
Existing methods often require extensive protein sequence and structural data.

Purpose of the Study:

To develop a computational method for predicting protein function changes due to single-point mutations using solely sequence information.
To evaluate the effectiveness of different local sequence features in prediction accuracy.

Main Methods:

Application of support vector machines (SVMs) for prediction.
Utilizing a large dataset from the Protein Mutant Database (PMD) for cross-validation.
Investigating three local sequence features: residue composition, hydrophobic interaction, and evolutionary property.

Main Results:

A novel substitution-matrix-based kernel SVM was developed, demonstrating speedy and accurate predictions.
The method effectively predicts protein function changes using only sequence data.
Analysis confirmed the impact of local sequence features on prediction accuracy.

Conclusions:

The developed SVM classifier provides an efficient and accurate tool for predicting the effects of single amino acid substitutions.
The findings highlight the utility of sequence-based features in understanding mutation impacts.
This method can aid in protein engineering and functional studies.