Related Experiment Video
Updated: Jun 22, 2026
06:45
Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes
Published on: August 9, 2024
Modeling and simulation of liquid-solid circulating fluidized bed ion exchange system for continuous protein recovery
Jahirul Mazumder1, Jingxu Zhu, Amarjeet S Bassi
1Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, Canada.
Biotechnology and Bioengineering
|May 26, 2009
Summary
A mathematical model for liquid-solid circulating fluidized beds (LSCFB) was developed to simulate continuous protein recovery. The model accurately predicts BSA adsorption and desorption, aiding system optimization.
Area of Science:
- Chemical Engineering
- Bioprocess Engineering
- Fluid Dynamics
Background:
- Liquid-solid circulating fluidized beds (LSCFB) offer integrated adsorption and desorption in a single unit.
- Continuous solid particle circulation between downcomer and riser columns is key to LSCFB operation.
- Efficient protein recovery is crucial in biopharmaceutical processes.
Purpose of the Study:
- To develop and validate a mathematical model for LSCFB systems.
- To simulate and analyze continuous protein recovery using LSCFB.
- To investigate the impact of operating parameters on BSA adsorption and desorption.
Main Methods:
- Developed a mathematical model assuming homogeneous fluidization.
- Incorporated hydrodynamics, adsorption-desorption kinetics, and liquid-solid mass transfer.
- Validated simulation results against experimental data for protein recovery.
Main Results:
- Simulation results demonstrated good agreement with experimental data for continuous protein recovery.
- A parametric sensitivity study identified key operating parameters influencing BSA capacity.
- The model provides insights into BSA adsorption and desorption dynamics within the LSCFB.
Conclusions:
- The developed mathematical model is effective for simulating LSCFB performance.
- The model can be extended to optimize LSCFB for various applications.
- This study enhances understanding of LSCFB for continuous bioprocessing.
Related Concept Videos
01:09Ion-Exchange Chromatography
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
01:17Ion Exchange
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
01:29Downstream Processing
Downstream processing begins once fermentation is complete and involves a series of steps to recover and purify products such as acids, vitamins, antibiotics, or proteins.Cell HarvestingFor example, for intracellular protein-based products, the first step is harvesting the cells. This is typically achieved using centrifugation or filtration to separate the cells from the liquid phase.Cell Disruption for Intracellular ProductsIf the target product is intracellular, the harvested cells must be...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:26Extracorporeal Removal of Drugs: Continuous Renal Replacement Therapy
Continuous Renal Replacement Therapy (CRRT) is an essential intervention for patients experiencing severe kidney dysfunction. This therapy offers a continuous mechanism for removing fluids and toxins from the bloodstream, leveraging the patient’s blood pressure to facilitate filtration through a specialized filter. This method contrasts with intermittent dialysis, providing a gentler and more consistent removal of waste products and excess fluid, which is particularly beneficial in critically...