Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simultaneous Functional Ultrasound, Intrinsic Optical Signal and Widefield Calcium Neuroimaging.

bioRxiv : the preprint server for biology·2026
Same author

Models of care for women living with HIV in Europe: A scoping review.

HIV medicine·2026
Same author

Skeletal correction in craniofacial microsomia: a case series and algorithm for management.

International journal of oral and maxillofacial surgery·2026
Same author

Late toxicity after peposertib-enhanced chemoradiation in rectal cancer patients managed with organ preservation.

Clinical and translational radiation oncology·2026
Same author

Comparative evaluation of the effects of 3D-printed camouflaged caterpillar, camouflaged alligator and conventional syringes on anxiety and behaviour in children.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2026
Same author

Metabotissugenic citrate biomaterials orchestrate bone regeneration via citrate-mediated signaling pathways.

Science advances·2025

Related Experiment Video

Updated: Jun 22, 2026

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

Self-diffraction in bacteriorhodopsin films for low power optical limiting.

D Narayana Rao, Chandra Yelleswarapu, Sri-Rajasekhar Kothapalli

    Optics Express
    |May 28, 2009
    PubMed
    Summary

    We developed a new low-power optical limiting method using bacteriorhodopsin (bR) films. This technique effectively protects eyes by clamping output light intensity to a safe level, regardless of input power.

    More Related Videos

    Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
    06:16

    Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

    Published on: April 25, 2019

    Super-resolution Imaging of the Bacterial Division Machinery
    08:47

    Super-resolution Imaging of the Bacterial Division Machinery

    Published on: January 21, 2013

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
    10:35

    Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

    Published on: May 29, 2018

    Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
    06:16

    Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

    Published on: April 25, 2019

    Super-resolution Imaging of the Bacterial Division Machinery
    08:47

    Super-resolution Imaging of the Bacterial Division Machinery

    Published on: January 21, 2013

    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Materials Science

    Background:

    • Optical limiting is crucial for protecting sensitive optical equipment and human vision from high-intensity laser damage.
    • Bacteriorhodopsin (bR) is a promising photochromic protein with potential applications in optical devices due to its unique light-induced transformations.
    • Existing optical limiting techniques often require high input energies or complex setups.

    Purpose of the Study:

    • To demonstrate a novel, low-power optical limiting technique utilizing self-diffraction in bacteriorhodopsin films.
    • To investigate the effectiveness of this method in clamping high input beam intensities to eye-safe levels.
    • To explore the relationship between material properties (M-state saturation intensity and lifetime) and optical limiting performance.

    Main Methods:

    • Utilized a continuous-wave (cw) Ar-Kr laser at 568 nm as the pump source.
    • Employed bacteriorhodopsin (bR) films for their photochromic properties.
    • Measured the first-order self-diffracted beam efficiency and output intensity across a range of input beam intensities (mW/cm² to W/cm²).

    Main Results:

    • Achieved optical limiting with an observed efficiency of approximately 0.01% for the first-order self-diffracted beam.
    • Successfully clamped the output beam intensity to a consistent eye-safe level of approximately 0.13 mW/cm².
    • Demonstrated that the threshold intensity for optical limiting is determined by the M-state saturation intensity of the bR, which can be tuned by film properties.

    Conclusions:

    • The self-diffraction technique in bR films offers an effective low-power optical limiting solution.
    • The performance of the optical limiter is directly linked to the photochromic characteristics of the bacteriorhodopsin material.
    • This method presents a viable approach for developing robust eye-safe optical systems.