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Shubham Mirg, Prameth Gaddale, Achutha Kumar

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|July 10, 2026

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Functional ultrasound (fUS) now maps brain blood volume with neuronal specificity. Integrating fUS with optical imaging reveals correlations between cerebral blood volume and calcium activity in mice.

Area of Science:

Neuroscience
Biomedical Engineering
Medical Imaging

Background:

Functional ultrasound (fUS) is a neuroimaging technique that measures cerebral blood volume (CBV).
However, fUS lacks molecular and neuronal specificity, limiting its application in understanding complex brain functions.
Integrating fUS with other imaging modalities can potentially overcome these limitations.

Purpose of the Study:

To enhance the specificity of functional ultrasound (fUS) by integrating it with optical imaging.
To investigate the correlation between fUS-derived CBV and neuronal activity.
To explore the utility of this integrated approach in studying neurovascular dysfunction in disease models.

Main Methods:

Simultaneously performed fUS and optical imaging in awake mice.

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Measured cerebral blood volume (CBV) using fUS.

Measured hemoglobin and neuronal calcium activity using optical imaging.

Derived hemodynamic response functions (HRFs) linking calcium activity to CBV.

Main Results:

fUS-derived CBV showed strong correlations with optically measured hemoglobin and neuronal calcium activity.
Hemodynamic response functions were successfully derived, linking calcium dynamics to CBV changes during both spontaneous and evoked neuronal activity.
The integrated approach was applied to a mouse glioblastoma model, demonstrating its potential for studying neurovascular dysfunction.

Conclusions:

Simultaneous fUS and optical imaging enhances the specificity of CBV measurements.
This integrated approach provides a powerful tool for investigating neurovascular coupling in various physiological and pathological conditions.
The method shows promise for studying complex neuropathologies like brain tumors.