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Updated: Jun 22, 2026
08:56
Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
A new triterpenoid from Brucea javanica.
Jian Hua Liu1, Jiang Jiang Qin, Hui Zi Jin
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Archives of Pharmacal Research
|May 28, 2009
Summary
Researchers isolated a new triterpenoid, bruceajavanin C, and eight other compounds from Brucea javanica. Several compounds showed mild inhibitory effects on nitric oxide (NO) production in immune cells.
Area of Science:
- Natural Product Chemistry
- Pharmacology
Background:
- Brucea javanica is a plant used in traditional medicine.
- Investigating its chemical constituents may yield novel therapeutic compounds.
Purpose of the Study:
- To isolate and identify compounds from the aerial parts of Brucea javanica.
- To evaluate the isolated compounds for their potential to inhibit nitric oxide (NO) production.
Main Methods:
- Phytochemical investigation of Brucea javanica aerial parts.
- Structure elucidation of isolated compounds using 2D-NMR spectroscopy.
- In vitro assay for nitric oxide inhibition in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
Main Results:
- A new triterpenoid, bruceajavanin C (1), was isolated and characterized.
- Eight known compounds (bruceosides A and B, bruceines D and E, yadanziosides A and G, and two pregnane derivatives) were also identified.
- Compounds 1, 3, 4, 5, and 6 demonstrated mild inhibitory effects on NO production.
Conclusions:
- The study successfully isolated and identified novel and known compounds from Brucea javanica.
- Several isolated compounds possess anti-inflammatory potential by inhibiting nitric oxide production, warranting further investigation.
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