Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecularly Imprinted Polymer-Based Chiral Stationary Phases for Chromatographic Enantioseparation.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Synthesis of the Abiraterone Derivatives 5α- and 5β-Δ<sup>1</sup>-Abiraterone and Structural Determination of an Unknown Metabolite in Human Serum Derived from Oral Abiraterone Acetate.

Chembiochem : a European journal of chemical biology·2025

Same author

Identification of novel metabolites of abiraterone in human serum and their metabolic pathways.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2023

Same author

A Phase II Trial of Abiraterone With Dutasteride for Second-Generation Antiandrogen- and Chemotherapy-Naïve Patients With Castration-Resistant Prostate Cancer.

Journal of clinical pharmacology·2022

Same author

Selected papers from the 31st International Symposium on Pharmaceutical and Biomedical Analysis (PBA2021).

Journal of pharmaceutical and biomedical analysis·2022

Same author

Progress in Chiral Stationary Phases Based on Proteins and Glycoproteins.

Chemical & pharmaceutical bulletin·2022

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Molecularly imprinted polymers as affinity-based separation media for sample preparation.

¹Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68, Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan. haginaka@mukogawa-u.ac.jp

Journal of Separation Science

|May 28, 2009

Summary

Molecularly imprinted polymers (MIPs) offer advanced affinity-based separation for sample preparation. This review covers MIP types, SPE modes, challenges, and diverse applications in various fields.

More Related Videos

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Related Experiment Videos

Last Updated: Jun 22, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Improved Polymerase Chain Reaction-restriction Fragment Length Polymorphism Genotyping of Toxic Pufferfish by Liquid Chromatography/Mass Spectrometry

Published on: September 20, 2016

Area of Science:

Analytical Chemistry
Materials Science

Background:

Molecularly imprinted polymers (MIPs) are crucial for selective molecular recognition.
Sample preparation is a critical step in many analytical processes.

Purpose of the Study:

To review MIPs as affinity-based separation media for sample preparation.
To discuss various MIP types, SPE modes, and their associated challenges and applications.

Main Methods:

Overview of molecularly imprinted particles and monoliths.
Discussion of different molecularly imprinted solid-phase extraction (MISPE) modes including online, offline, on-column extraction, SPME, and microextraction in packed syringe.
Analysis of challenges such as template leakage and aqueous incompatibility.

Main Results:

MIPs demonstrate versatility in sample preparation techniques.
Advantages and disadvantages of different MIP types and SPE modes are detailed.
Strategies for overcoming common MIP challenges are presented.

Conclusions:

MIPs are effective affinity-based separation media for sample preparation.
The review highlights the broad applicability of MIPs in pharmaceutical, food, bioanalytical, and environmental analyses.
Further research can optimize MIPs for enhanced performance and wider adoption.