Molecularly Imprinted Polymer-Based Chiral Stationary Phases for Chromatographic Enantioseparation

The synthesis of molecularly imprinted polymer (MIP)-based chiral stationary phases (CSPs) by precipitation polymerization or multistep swelling and polymerization and their application are described. Monodisperse, spherical MIP particles, whose diameters are about 5 μm, are prepared by those methods using a chiral template molecule. The obtained MIPs are suitable for enantioseparation of the target compound(s) in liquid chromatography (LC). Specifically, procedures for the synthesis of MIP-based chiral stationary phases by precipitation polymerization and multistep swelling and polymerization, respectively, for the enantioseparation of nicotine and warfarin, as well as the latter derivatives, are described.