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Updated: Oct 23, 2025

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Synthesis of Molecularly Imprinted Polymers by Two-Step Swelling and Polymerization
1Institute of Biosciences, Mukogawa Women's University, Nishinomiya, Japan. haginaka@mukogawa-u.ac.jp.
Abstract:
Synthesis of a molecularly imprinted polymer (MIP) by two-step swelling and polymerization is described. Monodisperse, spherical MIP particles, whose diameters are ca. 5-9μm, are prepared using a polystyrene particle as a shape template and dibutyl phthalate as an activating solvent. The obtained MIPs are suitable for separation media in liquid chromatography or solid-phase extraction media. Procedures for synthesis of MIPs and restricted access media (RAM)-MIP, packing of MIPs and RAM-MIPs, and application of MIPs and RAM-MIPs for selective separation and extraction of a target compound(s) are described.
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