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Published on: August 16, 2019
An immunoaffinity column with a monoclonal antibody as ligand for human follicle stimulating hormone
Marlene Zandian1, Alois Jungbauer
1Department of Biotechnology, University of Applied Life Sciences and Natural Resources, Vienna, Austria.
Abstract:
A detailed characterization regarding the chromatographic and biochemical properties of an immunoaffinity column for sample pretreatment using a follicle stimulating hormone (FSH) beta-unit specific mAb has been performed. A maximum binding capacity of approximately 300 microg/mL was determined. A K(D) value of 4.5x10(-8) M could be obtained in batch isotherm measurements. The dynamic binding capacity at 10% did not change when residence time was at least 6 min. High selectivity could be demonstrated using crude culture supernatant from FSH producing Chinese hamster ovary (CHO) cells. A great stability of the matrix was confirmed by performing 100 consecutive chromatographic runs without loss of capacity.
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