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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Updated: May 25, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

High throughput single nanoparticle spectroscopy.

David S Sebba¹, Dakota A Watson, John P Nolan

¹La Jolla Bioengineering Institute, 505 Coast Boulevard South, La Jolla, CA 92037, USA.

|May 29, 2009

Summary

This summary is machine-generated.

A new flow spectroscopy technique rapidly analyzes hundreds of nanoparticles per second. This high-throughput method enables detailed characterization of nanoparticle (NP) surface-enhanced resonant Raman scattering (SERRS) tags for nanoengineering applications.

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Area of Science:

Nanotechnology
Spectroscopy
Materials Science

Background:

Advancements in nanoengineered systems require efficient quantitative measurement techniques.
Current single nanoparticle characterization methods are slow and have low throughput, hindering progress.
High-throughput analysis is crucial for the engineering of nanoparticle (NP)-based systems.

Purpose of the Study:

To develop and demonstrate a high-throughput flow spectroscopy technique for nanoparticle analysis.
To enable rapid characterization of nanoparticle surface-enhanced resonant Raman scattering (SERRS) tags.
To improve the efficiency of nanoengineering workflows through advanced measurement capabilities.

Main Methods:

Implementation of a flow spectroscopy technique for analyzing individual nanoparticles.
Measurement of Rayleigh and Raman scattering from thousands of individual SERRS tags.
Utilizing high spectral resolution for detailed nanoparticle analysis.

Main Results:

Demonstrated a flow spectroscopy technique capable of analyzing hundreds of nanoparticles per second.
Enabled high-throughput characterization of nanoparticle SERRS tags based on brightness and uniformity.
Successfully analyzed thousands of individual tags, providing detailed insights into tag preparations.

Conclusions:

The developed flow spectroscopy technique offers a significant improvement in nanoparticle analysis throughput.
Rapid, high-resolution analysis of individual nanoparticles facilitates better nanoengineering.
This method is expected to benefit various fields within nanoengineering requiring precise nanoparticle characterization.