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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 22, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Chromatic confocal microscopy using supercontinuum light.

Kebin Shi, Peng Li, Shizhuo Yin

    Optics Express
    |May 29, 2009
    PubMed
    Summary
    This summary is machine-generated.

    A new chromatic confocal microscope uses supercontinuum white light for imaging. This system achieves a depth resolution under 1 micrometer, enabling detailed subsurface visualization.

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    10:28

    High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

    Published on: October 28, 2025

    Area of Science:

    • Optical microscopy
    • Photonics
    • Biomedical imaging

    Background:

    • Chromatic confocal microscopy offers optical sectioning capabilities.
    • Supercontinuum white light provides a broad spectral bandwidth for advanced imaging.

    Purpose of the Study:

    • To develop a novel chromatic confocal microscope system.
    • To utilize supercontinuum white light for enhanced depth resolution.

    Main Methods:

    • Generation of supercontinuum white light from a photonic crystal fiber.
    • Employing chromatic aberration of singlet lenses for spectral focusing.
    • Implementing a chromatic confocal microscopy setup.

    Main Results:

    • Demonstrated an effective depth scanning range of 7 micrometers.
    • Achieved a depth resolution of less than 1 micrometer (Full Width at Half Maximum).

    Conclusions:

    • The developed system offers high-resolution depth profiling.
    • This novel approach advances the capabilities of chromatic confocal microscopy.