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Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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Hyaline cartilage surface study with an environmental scanning electron microscope. An experimental study.

S Sastre1, S Suso, J M Segur

  • 1Orthopaedic Department of Hospital Clínic, C/Villarroel 170, 08036 Barcelona, Spain. ssastre@clinic.ub.es

Journal of Materials Science. Materials in Medicine
|May 29, 2009
PubMed
Summary
This summary is machine-generated.

Environmental scanning electron microscopy (ESEM) effectively images fresh osteochondral grafts. This technique allows for a validated classification system of chondral surface morphology, aiding graft evaluation.

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Area of Science:

  • Biomaterials Science
  • Orthopedic Surgery
  • Microscopy Techniques

Background:

  • Assessing the quality of fresh osteochondral grafts is crucial for successful transplantation.
  • Standard methods for evaluating chondral surface morphology have limitations.

Purpose of the Study:

  • To image the articular surface of fresh osteochondral grafts using environmental scanning electron microscopy (ESEM).
  • To evaluate and compare chondral surface morphology.
  • To develop a validated classification system for ESEM images of grafts.

Main Methods:

  • Osteochondral fragments from rabbit knee joints were analyzed.
  • One hundred images were acquired using ESEM.
  • Images were classified by two observers using a category system.
  • Kappa index and confidence intervals were calculated.

Main Results:

  • 62-72% of surfaces were even; 17-22% were hillocky, 12-16% knobbly.
  • 92-95% of surfaces showed no splits; 4-7% superficial, 1% deep.
  • 78-82% showed no lacunae; 17-20% filled, 1-2% empty.

Conclusions:

  • ESEM is a valuable tool for obtaining and classifying images of fresh osteochondral grafts.
  • The developed classification system aids in objective graft assessment.
  • ESEM provides detailed morphological insights into chondral surfaces.