Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evaluation of HER2 status and characteristics by next-generation sequencing in breast cancer.

Frontiers in molecular biosciences·2026

Same author

Identification and Analysis of the Mpp5Ab1-Interacting Protein in the Midgut of the <i>Colaphellus bowringi</i> Baly.

Toxins·2026

Same author

Biomechanical finite element methods study of a novel internal fixation system for lumbar spondylolysis.

Frontiers in bioengineering and biotechnology·2026

Same author

Mammogram geometric projection localization (MaGPL) for surgical body-surface localization of nonpalpable breast calcifications.

Gland surgery·2026

Same author

High-efficiency generation of structured light in a Ba(NO<sub>3</sub>)<sub>2</sub>-based cascaded Raman laser.

Optics letters·2026

Same author

Polarization dynamics of a diamond Raman laser governed by local stress and pump polarization perturbation.

Optics express·2026

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

MicroRNA and stem cell regulation.

Yulei Wang¹, Iain Russell, Caifu Chen

¹Life Technologies, Molecular Biology Systems Division, Foster City, CA 94404, USA.

Current Opinion in Molecular Therapeutics

|May 30, 2009

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are key regulators in stem cell biology, influencing embryonic, adult, and cancer stem cells (CSCs). Understanding these roles advances regenerative medicine and cancer treatment strategies.

More Related Videos

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Related Experiment Videos

Last Updated: Jun 22, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Published on: March 8, 2019

Area of Science:

Stem cell biology
Molecular biology
Cancer research

Background:

Stem cells possess self-renewal and multipotency, crucial for development and tissue maintenance.
Cancer stem cells (CSCs) exhibit stem-like properties, driving tumor growth and drug resistance.
MicroRNAs (miRNAs) are critical regulators of gene expression and stem cell function.

Purpose of the Study:

To review recent advances in understanding the role of miRNAs in regulating various stem cell types.
To highlight the significance of miRNAs in embryonic stem cells, adult stem cells, and CSCs.
To explore the potential of miRNA research in regenerative medicine and cancer therapy.

Main Methods:

Literature review of recent scientific publications.
Synthesis of current knowledge on miRNA-mediated regulation in stem cells.
Analysis of the implications of miRNAs in stem cell biology and disease.

Main Results:

miRNAs play integral roles in regulating self-renewal and differentiation of embryonic and adult stem cells.
Dysregulation of specific miRNAs is implicated in the maintenance and function of CSCs.
miRNAs are involved in both promoting and suppressing CSC characteristics.

Conclusions:

miRNAs are fundamental regulators of stem cell pluripotency, differentiation, and behavior.
Targeting miRNAs offers a promising therapeutic strategy for regenerative medicine and cancer treatment.
Further research into miRNA-stem cell interactions will unlock new avenues for disease intervention.