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Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA
S Shimada1, S Kitayama, C L Lin
1Laboratory of Molecular Neurobiology, National Institute on Drug Abuse, Baltimore, MD 21224.
Science (New York, N.Y.)
|October 25, 1991
Abstract:
A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.