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Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
Published on: January 15, 2018
Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular
Jine Wang1, Taicheng Duan, Linlin Sun
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Abstract:
In this study, lectin-conjugated gold nanoparticles (GNPs) were prepared by standard biotin-streptavidin chemistry. The lectin-conjugated GNPs can be used as an indicator for studying the interaction of lectin with glycosyl complex on living cellular surfaces due to the high affinity of the lectin with saccharides. The interactions of two well-known lectins (Ricinus communis agglutinin and concanavalin A) and three different cell lines (HeLa, 293, and 293T) were selected here to establish this assay. Highly binding affinity of R. communis agglutinin with cells was demonstrated by conventional microscopic and UV-visible spectroscopic studies. In addition, the binding process can be inhibited by galactose, giving further proof of the binding mechanism.

