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Updated: Jun 22, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
Published on: June 20, 2010
Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
Carl Grey1, Per Edebrink, Maria Krook
1BioPR&D, AstraZeneca R&D, SE-15185 Södertälje, Sweden. carl.grey@biotek.lu.se
Abstract:
In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. In addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method.
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