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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
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CHLOROPLAST LIPID-REMODELING PROTEIN 23 functions during cold acclimation in Arabidopsis thaliana
Wing Tung Lo1, Denise Winkler1, Maximilian Münch1
1Plant Molecular Biology, LMU München, Großhaderner Str. 2-4, Planegg-Martinsried 82152, Germany.
Plant Physiology
|February 18, 2026
Summary
Chloroplast Lipid Remodeling Protein 23 (CLRP23) is vital for plant cold acclimation. CLRP23 helps maintain photosynthesis and membrane fluidity by interacting with chloroplast lipids under cold stress.
Area of Science:
- Plant Physiology
- Molecular Biology
- Biochemistry
Background:
- Cold acclimation is essential for plants to survive low temperatures.
- Lipid remodeling in chloroplast membranes maintains fluidity and integrity during cold stress.
- Specific proteins mediating lipid remodeling in chloroplasts remain largely uncharacterized.
Purpose of the Study:
- To investigate the function of Chloroplast Lipid Remodeling Protein 23 (CLRP23) in plant cold acclimation.
- To determine the localization and lipid-binding properties of CLRP23.
- To elucidate the role of CLRP23 in integrating membrane and metabolic responses to cold.
Main Methods:
- Subcellular fractionation and protease protection assays for protein localization.
- Analysis of physiological performance and lipid composition in CLRP23-deficient mutants under cold stress.
- Lipid overlay assays, in silico docking, transcriptomic, and proteomic analyses.
Main Results:
- CLRP23 is localized to the inner chloroplast envelope, oriented toward the intermembrane space.
- CLRP23-deficient mutants exhibit impaired photosynthesis and altered galactolipid profiles under cold stress.
- CLRP23 directly interacts with chloroplast lipids, including galactolipids, and influences broader cold-responsive pathways.
Conclusions:
- CLRP23 plays a significant role in chloroplast lipid remodeling during cold acclimation.
- CLRP23 contributes to maintaining photosynthetic function and membrane integrity under cold conditions.
- CLRP23 integrates membrane lipid dynamics with metabolic adjustments for effective cold acclimation in plants.
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