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Updated: Jun 22, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
Raman-assisted X-ray crystallography for the analysis of biomolecules
Dominique Bourgeois1, Gergely Katona, Eve de Rosny
1Institut de Biologie Structurale Jean-Pierre Ebel, 41 Rue Jules Horowitz, 38027, Grenoble Cedex, France. dominique.bourgeois@ibs.fr
Abstract:
In this chapter, we describe Raman microspectrophotometry applied to crystals of biomolecules. Raman spectra collected in crystallo provide structural information highly complementary to X-ray diffraction, relate the crystalline state to the solution state, and allow the identification of ligand-bound or intermediate states of macromolecules. Nonresonant Raman spectroscopy is particularly suitable to the study of macromolecular crystals, and therefore applies to a wide range of noncolored crystalline proteins. Practical issues related to the investigation of crystals by Raman microspectrophotometry are reviewed, and the current limitations are highlighted.
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