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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Updated: Jun 22, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Comparative genomic hybridization by representational oligonucleotide microarray analysis.

Robert Lucito1, James Byrnes

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2009
PubMed
Summary
This summary is machine-generated.

Genomic alterations are the root cause of cancer, leading to changes in gene expression and cell phenotype. Representational oligonucleotide microarray analysis (ROMA) detects these genomic deletions and amplifications to identify cancer genes.

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Last Updated: Jun 22, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
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13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

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Area of Science:

  • Genomics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Cancer originates from genomic alterations affecting gene expression and cell phenotype.
  • Complex gene expression changes can obscure primary genomic lesions responsible for carcinogenesis.
  • Genomic alterations range from point mutations and indels to copy number variations and translocations, alongside epigenetic modifications.

Purpose of the Study:

  • To describe a method for detecting genomic deletions and amplifications in cancer.
  • To identify underlying cancer genes driving tumor growth.
  • To overcome the obscuring effects of complex gene expression cascades in cancer.

Main Methods:

  • Utilizes a variation of comparative genomic hybridization.
  • Employs Representational Oligonucleotide Microarray Analysis (ROMA).
  • Surveys reduced-complexity representations of tumor genomic DNA.

Main Results:

  • ROMA effectively discovers deletions and amplifications in tumor genomes.
  • Identifies key cancer genes altered by these genomic events.
  • Provides insights into the primary genomic lesions driving carcinogenesis.

Conclusions:

  • Genomic alterations are fundamental to cancer development.
  • ROMA is a valuable tool for detecting copy number variations in cancer.
  • Identifying these alterations aids in understanding and potentially targeting cancer genes.