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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 22, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Methylation analysis by microarray.

Daniel E Deatherage1, Dustin Potter, Pearlly S Yan

  • 1Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2009
PubMed
Summary
This summary is machine-generated.

Differential methylation hybridization (DMH) is a screening tool for DNA methylation. This study details a robust DMH protocol with quality control for reliable methylation profiling.

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DNA Methylation: Bisulphite Modification and Analysis

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12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology Techniques

Background:

  • DNA methylation is a critical epigenetic modification influencing gene expression.
  • High-throughput screening methods are needed for comprehensive methylation analysis.
  • Differential methylation hybridization (DMH) offers a microarray-based approach to profile DNA methylation.

Purpose of the Study:

  • To describe a detailed and reproducible Differential Methylation Hybridization (DMH) protocol.
  • To establish quality control (QC) measures for reliable DNA methylation profiling.
  • To outline data analysis procedures for DMH, including quantification and statistical inference.

Main Methods:

  • Utilized methylation-sensitive restriction enzymes to digest genomic DNA.
  • Hybridized differentially methylated DNA fragments to a CpG island microarray.
  • Implemented step-by-step laboratory procedures with defined quality control checkpoints.

Main Results:

  • A comprehensive DMH protocol is presented, enabling the profiling of DNA methylation across 27,800 CpG islands.
  • Quality control points are integrated to identify and rectify suboptimal sample read-outs.
  • Detailed methods for image quantification, background correction, and statistical analysis are provided.

Conclusions:

  • The described DMH protocol provides a standardized and reliable method for high-throughput DNA methylation screening.
  • Integrated quality control ensures the accuracy and reproducibility of differential methylation profiles.
  • The protocol facilitates robust data analysis for both exploratory and inferential studies of DNA methylation.