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Updated: Jun 22, 2026
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Methylated DNA Immunoprecipitation
Published on: January 2, 2009
Methylation analysis by DNA immunoprecipitation (MeDIP).
Emily A Vucic1, Ian M Wilson, Jennifer M Campbell
1British Columbia Cancer Research Centre, BC, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2009
Summary
Epigenetic alterations, like CpG island hypermethylation, are key in cancer and developmental diseases. Methylated DNA immunoprecipitation (MeDIP) helps profile the methylome for disease gene discovery and understanding gene silencing patterns.
Area of Science:
- Epigenetics and Molecular Biology
- Genomics and Bioinformatics
Background:
- Epigenetic dysregulation, particularly CpG island hypermethylation leading to gene silencing, is prevalent in human cancers and developmental disorders.
- Understanding these epigenetic alterations is crucial for identifying disease-associated genes and pathways.
Purpose of the Study:
- To present a detailed protocol for Methylated DNA Immunoprecipitation (MeDIP).
- To enable comparative methylome profiling for disease gene discovery.
- To facilitate genome-wide analysis of epigenetic alterations.
Main Methods:
- Methylated DNA Immunoprecipitation (MeDIP) using 5'-methylcytosine-specific antibodies to isolate methylated DNA fragments.
- Locus-specific analysis via PCR assay.
- Genome-wide analysis using comparative genomic hybridization (CGH) against a non-enriched sample.
- Hybridization of MeDIP DNA to a whole-genome tiling path BAC array.
Main Results:
- The protocol allows for the enrichment of methylated DNA.
- MeDIP enables both locus-specific and genome-wide profiling of DNA methylation patterns.
- Successful hybridization of MeDIP DNA to BAC arrays for comprehensive analysis.
Conclusions:
- MeDIP is an effective technique for comparative methylome profiling.
- This method is essential for identifying epigenetic alterations and understanding gene silencing in disease.
- The described protocol facilitates global analysis of the methylome for disease gene discovery.
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