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Updated: Jun 22, 2026
Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
Rhesus macaque blastocysts resulting from intracytoplasmic sperm injection of vacuum-dried spermatozoa
Stuart A Meyers1, Ming-Wen Li, Allen C Enders
1Department of Anatomy, Physiology, and Cell Biology, University of California, Davis, CA 95616, USA. smeyers@ucdavis.edu
Background:
Sperm desiccation is an attractive approach for sperm preservation. In this study, we examined the feasibility and efficiency of intracytoplasmic sperm injection using vacuum-dried rhesus macaque sperm in CZB medium supplemented with 10% fetal bovine serum.
Methods:
A total of 109 MII oocytes were injected with 69 fresh ejaculated sperm and 40 vacuum-dried sperm.
Results:
Cleavage occurred in 97% of oocytes injected with fresh, motile sperm and in 88% of oocytes injected with vacuum-dried sperm. Of the cleaved oocytes, 68% fresh sperm-injected oocytes and 74% of dried sperm-injected oocytes developed to the compact morula stage. Blastocyst development was comparable between fresh-injected (16%) and vacuum-dried-injected (17%) oocytes. Differences between treatment groups were not significant. Transmission electron microscopic observation of the blastocysts indicated no detectable differences between fresh sperm and dried sperm-derived embryos.
Conclusions:
We conclude that vacuum-dried rhesus macaque sperm are capable of inducing fertilization and development of pre-implantation embryos when sperm were dried under vacuum and microinjected into normal viable oocytes.
