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Related Experiment Video

Updated: Jun 22, 2026

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility
07:35

In Vivo Vascular Injury Readouts in Mouse Retina to Promote Reproducibility

Published on: April 21, 2022

A pilot study using ROPtool to measure retinal vascular dilation.

David K Wallace1, Sharon F Freedman, Zheen Zhao

  • 1Department of Ophthalmology, Duke University, Durham, North Carolina, USA. david.wallace@duke.edu

Retina (Philadelphia, Pa.)
|June 4, 2009
PubMed
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ROPtool now accurately measures retinal vascular width, aiding in the diagnosis of plus disease in retinopathy of prematurity (ROP). This technology reduces subjectivity in assessing retinal vascular dilation.

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Computational Biology

Background:

  • Plus disease, characterized by abnormal retinal vascular dilation and tortuosity, is a primary indicator for laser treatment in retinopathy of prematurity (ROP).
  • ROPtool is a validated computer program for measuring retinal arteriolar tortuosity.
  • The study aimed to evaluate ROPtool's new capability to measure retinal vascular width (dilation).

Purpose of the Study:

  • To assess the accuracy of ROPtool's newly developed measurement for retinal vascular width.
  • To determine if ROPtool can objectively quantify retinal vascular dilation in retinopathy of prematurity.

Main Methods:

  • ROPtool measured the width of 154 blood vessels in 20 high-quality RetCam images from premature infants.
  • Measurements were compared against the mean scores of two expert authors who graded retinal vascular dilation on a 10-point scale.

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Last Updated: Jun 22, 2026

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Published on: April 21, 2022

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
12:28

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Published on: March 12, 2022

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Main Results:

  • ROPtool demonstrated a strong correlation (r = 0.80) with expert grading of retinal vascular dilation.
  • Areas under the receiver operating characteristic curves for identifying plus disease and pre-plus disease were 0.93 and 0.90, respectively.
  • The tool achieved 89% sensitivity and 83% specificity in diagnosing dilation sufficient for plus disease.

Conclusions:

  • ROPtool accurately measures retinal vascular width in high-quality RetCam images, complementing its tortuosity measurement.
  • This technology has the potential to eliminate subjectivity in the assessment of plus disease.
  • Automated measurement of retinal vascular dilation can improve ROP diagnosis and treatment decisions.