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Updated: Jun 22, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Efficient solid-phase synthesis of oligodeoxynucleotides having a 5'-terminal 2,2,7-trimethylguanosine pyrophosphate
Akihiro Ohkubo1, Kenji Sasaki, Yasuhiro Noma
1Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan. eloizido@ucy.ac.cy
Abstract:
A 5'-terminal 2,2,7-trimethylguanosine (TMG) cap structure plays an important role in transport of TMG-capped snRNAs from the cytoplasm to the nucleus in living cells. This property can be expected to be used for carrying artificial nucleic acids into the nucleus in gene therapy. In this paper, a new TMG-capping reagent 1 was developed and used for the synthesis of oligodeoxynucleotides having a 5'-terminal TMG-capped pyrophosphate linkage (m(3)(2,2,7)G(5')pp-DNA) lacking one phosphate group using a silyl-type linker on polymer supports.
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