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Chromosomal numerical aberrations in oral lichen planus.
N Yarom1, T Shani, N Amariglio
1Department of Oral and Maxillofacial Surgery, The Chaim Sheba Medical Center, Tel Hashomer, Israel.
Journal of Dental Research
|June 5, 2009
Summary
Oral lichen planus (OLP) can show chromosomal instability. Detecting aneuploid cells using morphology and FISH analysis helps predict OLP
Area of Science:
- Oral pathology
- Cytogenetics
- Cancer research
Background:
- Oral lichen planus (OLP) is controversial regarding its malignant potential.
- Accurate prediction of malignant transformation in OLP is clinically significant.
Purpose of the Study:
- To detect chromosomal numerical aberrations in oral lichen planus (OLP) cells.
- To assess the utility of aneuploid cell detection for predicting malignant potential in OLP.
Main Methods:
- Brush samples from OLP-affected mucosa and controls were analyzed.
- Morphological analysis combined with fluorescent in situ hybridization (FISH) using chromosome 2 and 8 centromeric probes.
- A cut-off of 1.1% aneuploid cells was established.
Main Results:
- Aneuploid cells were found in 28.1% of OLP patients (16/57).
- Over 5% aneuploid cells were observed in 17.5% of OLP patients (10/57).
- Aneuploid cells were detected in clinically normal mucosa of 7 OLP patients.
Conclusions:
- Oral lichen planus (OLP) is associated with increased chromosomal instability.
- Combined morphological and FISH analysis of brush samples can improve specificity in predicting OLP's malignant potential.
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