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Updated: Jun 22, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Dy3+ and Nd3+ induced genetic mutation of bacillus alpha-amylase
Dongyan Zhang1, Jianhua Hu, Bater Siqin
1School of Chemical Engineering, Department of Biological Engineering, Inner Mongolia University of Technology, Hohhot, China. xym@imut.edu.cn
Abstract:
After treatment with DyCl(3) and NdCl(3), two strains of mutated bacilli showing increased alpha-amylase activity were isolated. The alpha-amylase genes were amplified, cloned, and sequenced. Sequencing revealed that there were either 11 or 14 bases altered in the two genes. These alterations took the form of base substitutions, and transversion was more common than transition. Based on these results, it was concluded that the rare earth compounds DyCl(3) and NdCl(3) were mutagens.
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