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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Compact Quantum Dots for Single-molecule Imaging

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Published on: October 9, 2012

Single molecule fluorescence in rectangular nano-apertures.

Jérôme Wenger, Pierre-François Lenne, Evgueni Popov

|June 6, 2009

Summary

Fluorescence Correlation Spectroscopy reveals enhanced molecular fluorescence in nanoapertures. Tailored evanescent fields boost fluorescence rates, primarily due to near-field excitation effects within the subwavelength structures.

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Last Updated: Jun 22, 2026

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Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Area of Science:

Nanophotonics
Spectroscopy
Physical Chemistry

Background:

Fluorescence Correlation Spectroscopy (FCS) probes molecular dynamics.
Subwavelength apertures in metal films offer unique optical properties.
Metal surfaces can influence molecular photophysics.

Purpose of the Study:

Investigate fluorescent molecules diffusing in rectangular nanoapertures.
Explore the tunability of excitation fields within these apertures.
Quantify fluorescence enhancement and its origin.

Main Methods:

Utilized Fluorescence Correlation Spectroscopy (FCS).
Fabricated subwavelength rectangular apertures in Aluminium films.
Manipulated excitation field (propagating vs. evanescent) within apertures.

Main Results:

Observed reduced molecular lifetime near the metal surface.
Demonstrated significant fluorescence rate enhancement per molecule with tailored evanescent fields.
Attributed enhancement to near-field excitation within the nanoaperture.

Conclusions:

Nanoapertures enable control over observation volume and excitation fields.
Evanescent excitation fields within nanoapertures significantly enhance detected fluorescence.
Near-field optical effects are crucial for fluorescence enhancement in these nanostructures.