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Updated: Aug 7, 2026

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Published on: June 5, 2020
Biophysics of organoids
Vanessa Weichselberger1, Gareth Moore2, Sham Tlili3
1Aix Marseille Univ, CNRS, IBDM (UMR 7288), Turing Centre for Living Systems, Marseille, France; European Molecular Biology Laboratory, EMBL Barcelona, Dr. Aiguader 88, PRBB Building, 08003 Barcelona, Spain.
Abstract:
Organoids, 3D in vitro structures derived from embryonic or adult stem cells, offer powerful models for studying tissue patterning, development, morphogenesis, organ physiology, and disease. These systems replicate biological processes, such as cell differentiation, symmetry breaking, and tissue organization, while revealing species-specific developmental variations. Biophysical factors, such as extracellular matrix composition, cell motility, tissue flows, and stiffness, interact with biochemical signals to drive organoid formation, revealing complex multiscale phenomenon during growth, patterning, and homeostasis. Physics-based approaches provide a framework to understand these processes from first principles. In recent years, a growing community of researchers has been exploring what can be termed the "biophysics of organoids." This review covers a broad range of approaches-mechanical, kinetic, information-based, statistical, and artificial intelligence (AI)-driven-to study organoid development, offering insights into organogenesis, disease modeling, and regenerative medicine.
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