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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 22, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Optical scattering spectroscopy by using tightly focused supercontinuum.

Peng Li, Kebin Shi, Zhiwen Liu

    Optics Express
    |June 6, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We analyzed light scattering from a single sphere using advanced optical techniques. Our experimental findings closely matched theoretical predictions from Mie scattering theory.

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    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
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    Last Updated: Jun 22, 2026

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
    15:06

    Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

    Published on: January 3, 2016

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
    07:55

    High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

    Published on: September 22, 2017

    Area of Science:

    • * Physics
    • * Optics
    • * Materials Science

    Background:

    • * Understanding light-matter interactions is crucial in various scientific fields.
    • * Optical scattering provides insights into the properties of microscopic objects.
    • * Mie scattering theory is a fundamental model for light scattering by spheres.

    Purpose of the Study:

    • * To analyze the forward optical scattering spectra of a single spherical object.
    • * To validate theoretical models with experimental data for light scattering.
    • * To investigate the application of supercontinuum light sources in scattering analysis.

    Main Methods:

    • * Illumination of a single spherical object with tightly focused white light supercontinuum.
    • * Analysis of forward optical scattering spectra.
    • * Application of angular spectrum decomposition and Mie's scattering theory for calculations.

    Main Results:

    • * Detailed analysis of forward optical scattering spectra was performed.
    • * Experimental results demonstrated strong agreement with theoretical calculations.
    • * The study successfully validated Mie scattering theory for the observed phenomena.

    Conclusions:

    • * The presented analysis confirms the validity of Mie scattering theory for spherical objects.
    • * Tightly focused supercontinuum light is effective for probing scattering properties.
    • * The methodology provides a reliable approach for characterizing micro-objects optically.