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Updated: Jun 22, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
A new strategy to apply Bacillus subtilis MA139 for the production of solid-state fermentation feed
1State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China.
Aim:
The study investigated the potential of using Bacillus subtilis MA139 in combination with Lactobacillus fermentum and Saccharomyces cerevisae to produce solid-state fermentation feed.
Methods And Results:
In a pure fermentation, B. subtilis MA139 was able to grow and synthesize antimicrobial substances at temperatures from 25 to 37 degrees C and at a pH from 5.0 to 9.0. Subsequently, B. subtilis MA139, Lact. fermentum and S. cerevisae were used as starter strains co-inoculated in unsterilized substrate (feed-grade soybean meal and wheat bran). Following 10 days of fermentation in a newly developed plastic bag equipped with a one-way valve, lactic acid bacteria and Bacillus became the predominant strains while S. cerevisae cells decreased slightly. Enterobacteriaceae (Escherichia coli K88 and Salmonella typhimurium) were not detected.
Conclusions:
Use of B. subtilis MA139 as a starter strain co-inoculated with S. cerevisae and Lact. fermentum successfully controlled the growth of enterobacteriaceae.
Significance And Impact Of The Study:
This study provided a facile and low-cost way to produce solid-state fermentation feed.
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