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Related Experiment Video

Updated: Jun 22, 2026

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

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[Polyclonal antibody preparation and expression pattern analysis of CARP].

Guo-da Ma¹, Yu-dong Cui, Fan Weng

¹College of Life Science and Technology, August First Land Reclamation University, Daqing 163319, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology

|June 9, 2009

Summary

Researchers developed a CARP antibody and found CARP is specifically expressed in the mouse heart, primarily in the nucleus of rat cardiac cells, aiding future functional studies.

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Area of Science:

Molecular Biology
Immunology
Cardiovascular Research

Context:

CARP (Cardiac Anodal Repolarization Protein) function is not fully understood.
Developing specific antibodies is crucial for studying protein expression and localization.

Purpose:

To prepare a specific antibody against CARP.
To analyze the expression pattern of CARP in cardiac tissues.

Summary:

A CARP antibody was generated using recombinant protein expressed in E. coli and subsequent immunization.
CARP expression was confirmed in mouse heart tissue.
Immunofluorescence revealed CARP is predominantly localized in the nucleus of rat cardiac primary cells.

Impact:

The generated CARP antibody provides a valuable tool for further research.
This study lays the groundwork for investigating the functional role of CARP in cardiac biology.