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Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
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Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
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Related Experiment Video

Updated: Jun 22, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Intercellular adhesion molecule-1 clusters during osteoclastogenesis.

Veerle Bloemen1, Teun J de Vries, Ton Schoenmaker

  • 1Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Research Institute MOVE, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.

Biochemical and Biophysical Research Communications
|June 9, 2009
PubMed
Summary
This summary is machine-generated.

Intercellular adhesion molecule-1 (ICAM-1) on osteoblasts initially aids osteoclast precursor adhesion. Later, ICAM-1 on osteoclast precursors, clustered and linked to F-actin, suggests a role in cell movement during osteoclastogenesis.

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Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Published on: March 22, 2024

Area of Science:

  • Cell Biology
  • Biochemistry
  • Immunology

Background:

  • Osteoblast and osteoclast precursor adhesion is crucial for bone remodeling.
  • Intercellular adhesion molecule-1 (ICAM-1) on osteoblasts interacts with leukocyte function-associated antigen-1 (LFA-1) on osteoclast precursors.
  • The dynamic expression and role of ICAM-1 on osteoclast precursors during osteoclastogenesis are not well understood.

Purpose of the Study:

  • To investigate the temporal expression of ICAM-1 on human osteoblast-like cells and osteoclast precursors in co-culture.
  • To elucidate the localization and potential function of ICAM-1 during osteoclastogenesis.

Main Methods:

  • Co-culture of human osteoblast-like cells and osteoclast precursors.
  • Analysis of ICAM-1 protein expression over time using immunofluorescence or flow cytometry.
  • Assessment of ICAM-1 localization and association with cytoskeletal components like F-actin.

Main Results:

  • Osteoblast-like cells showed decreased ICAM-1 expression over time.
  • Osteoclast precursors exhibited a significant increase in ICAM-1 expression.
  • ICAM-1 on osteoclast precursors formed basolateral clusters associated with F-actin, persisting for several days.

Conclusions:

  • Osteoblastic ICAM-1 primarily mediates initial osteoclast precursor adhesion.
  • Clustered ICAM-1 on osteoclast precursors, linked to F-actin, suggests a role in cell migration during later stages of osteoclastogenesis.