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Updated: Jun 22, 2026

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published on: July 18, 2011
Glutathione participates in the regulation of mitophagy in yeast
Ingrid Bhatia Kissová1, Nadine Camougrand
1Comenius University, Department of Biochemistry, Bratislava, Slovak Republic.
Abstract:
The term mitophagy refers to the selective removal of mitochondria by autophagy. This process is very active in lactate-grown yeast cells during nitrogen starvation. Among different antioxidants tested, only N-acetyl L-cysteine (NAC) prevents mitophagy in lactate-grown nitrogen-starved cells and its effect is not related to its scavenging properties, but rather to the fueling effect of the glutathione pool. In these conditions, glutathione regulates mitophagy independently of general autophagy.
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