Towards optimum sample-probe-spectrometer system design by adjusting receiving fiber end face position and...

Area of Science:

• Optical spectroscopy
• Biomedical instrumentation

Background:

• Accurate fluorescence measurements are crucial for analyzing thin membrane samples.
• Fiber optic probes coupled with spectrometers are commonly used for in-situ analysis.
• System performance is often limited by probe design and spectrometer coupling.

Purpose of the Study:

• To investigate an optimized two-fiber probe design for enhanced fluorescence spectroscopy.
• To improve both spectrum resolution and fluorescence collection efficiency simultaneously.
• To maximize the performance of a sample-probe-spectrometer system.

Main Methods:

• Utilizing a two-fiber probe interrogated by a spectrometer.
• Investigating fluorescence emitted from a thin membrane layer.
• Theoretically and experimentally analyzing the effect of separating fiber probe faces.

Main Results:

• Separating the receiving and illuminating fiber faces simultaneously enhances spectrum resolution and fluorescence collection.
• The optimized system collects more emitted rays within the spectrometer's acceptance angle.
• A relative collection efficiency increase of up to 63% was observed with optimal sample positioning.

Conclusions:

• A novel two-fiber probe design significantly improves fluorescence spectroscopy performance.
• Optimized probe geometry and sample positioning are critical for maximizing spectral data acquisition.
• This approach offers a more efficient method for analyzing membrane fluorescence.