Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CAF-derived PAI-1 serves as an EGFR ligand and fuels KRAS-mutant pancreatic cancer.

Nature communications·2026
Same author

Interfacial Charge Transfer Directing Intermolecular Hydrogen Transfer for On-Surface C-H Activation.

Journal of the American Chemical Society·2026
Same author

A new plant SUMO ligase, MPEL1, synergizes with MAPK16 to regulate resistance against Fusarium pathogens.

The EMBO journal·2026
Same author

Distribution and fracture characteristics of major lithologic associations in the Upper Paleozoic Ordos Basin.

Scientific reports·2026
Same author

Plant-Derived Nanovesicles: Resolving Conceptual Confusion, Overcoming Isolation Challenges, and Advancing Translational Potential.

International journal of nanomedicine·2026
Same author

Biological control of a novel strain Bacillus velezensis BFWR11 against tomato early blight.

Antonie van Leeuwenhoek·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jun 22, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Single mode optofluidic distributed feedback dye laser.

Zhenyu Li, Zhaoyu Zhang, Teresa Emery

    Optics Express
    |June 9, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study demonstrates single-frequency lasing from organic dye solutions on a poly(dimethylsiloxane) (PDMS) chip. The microfabricated dye laser offers a compact, inexpensive coherent light source for integrated optics and microfluidics.

    More Related Videos

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
    08:48

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

    Published on: November 22, 2019

    Fluorescence detection methods for microfluidic droplet platforms
    14:16

    Fluorescence detection methods for microfluidic droplet platforms

    Published on: December 10, 2011

    Related Experiment Videos

    Last Updated: Jun 22, 2026

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
    08:48

    Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

    Published on: November 22, 2019

    Fluorescence detection methods for microfluidic droplet platforms
    14:16

    Fluorescence detection methods for microfluidic droplet platforms

    Published on: December 10, 2011

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Microfluidics

    Background:

    • Organic dye lasers offer tunable coherent light sources.
    • Microfabrication techniques are crucial for developing compact laser devices.
    • Integrated optics and microfluidics require miniaturized and cost-effective light sources.

    Purpose of the Study:

    • To demonstrate single-frequency lasing from organic dye solutions on a monolithic poly(dimethylsiloxane) (PDMS) elastomer chip.
    • To develop a compact and inexpensive coherent light source for microfluidics and integrated optics.

    Main Methods:

    • Fabrication of a monolithic PDMS chip with a single-mode liquid core/PDMS cladding channel waveguide.
    • Integration of a phase-shifted 15th order distributed feedback (DFB) structure within the waveguide.
    • Utilizing Rhodamine 6G dye solution as the gain medium, pumped by nanosecond 532nm Nd:YAG laser pulses.

    Main Results:

    • Achieved a threshold pump fluence of approximately 0.8 mJ/cm(2).
    • Demonstrated stable single-mode operation at pump levels up to ten times the threshold.
    • The microfabricated dye laser operates across a broad spectral range from near UV to near IR.

    Conclusions:

    • Single-frequency lasing is successfully achieved in a microfabricated organic dye laser on a PDMS chip.
    • The developed device is a compact, inexpensive, and efficient coherent light source.
    • This technology holds promise for applications in microfluidics and integrated optics.