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Transgenic Organisms00:53

Transgenic Organisms

Overview

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Related Experiment Video

Updated: Jun 22, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Generation of transgenic frogs.

Jana Loeber1, Fong Cheng Pan, Tomas Pieler

  • 1Department of Developmental Biochemistry, University of Goettingen, Goettingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2009
PubMed
Summary

Generating transgenic frogs (Xenopus) is crucial for studying gene function in development and disease. The meganuclease method offers a simple, efficient technique for creating these valuable research models.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Transgenic animals are vital for understanding gene function in development and disease.
  • Xenopus laevis is a well-established vertebrate model organism in developmental biology research.
  • Various methods exist for creating transgenic Xenopus.

Purpose of the Study:

  • To describe the meganuclease method for generating transgenic Xenopus.
  • To highlight the simplicity, efficiency, and applicability of this technique.

Main Methods:

  • Utilizing the commercially available endonuclease I-SceI (meganuclease).
  • Coinjection of foreign DNA and the meganuclease into fertilized Xenopus eggs.
  • Employing the meganuclease method for Xenopus laevis and Xenopus tropicalis.

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Related Experiment Videos

Last Updated: Jun 22, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus
05:34

Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus

Published on: February 1, 2018

Generating Retinal Injury Models in Xenopus Tadpoles
09:29

Generating Retinal Injury Models in Xenopus Tadpoles

Published on: October 13, 2023

Main Results:

  • Successful integration of foreign DNA into the Xenopus genome.
  • Observed tissue-specific gene expression in the generated transgenic frogs.
  • Demonstrated germline transmission of the integrated foreign DNA.

Conclusions:

  • The meganuclease method is a technically simple and efficient approach for Xenopus transgenesis.
  • This method facilitates the analysis of gene function in Xenopus development and disease models.
  • The technique is applicable to both Xenopus laevis and Xenopus tropicalis, expanding research capabilities.