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Updated: Jun 22, 2026
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
Surgical techniques for the generation of mutant mice
1Centre for Mouse Genetics, Institute for Genetics, University of Cologne, Koeln, Germany. Anne.Plueck@uni-koeln.de
Methods in Molecular Biology (Clifton, N.J.)
|June 9, 2009
Summary
This chapter details embryo reimplantation into foster mice for development. It covers surgical techniques for transferring embryos, including blastocyst and oocyte stages, and methods for creating pseudopregnant females.
Area of Science:
- Developmental Biology
- Reproductive Science
Background:
- Previous chapters covered chimeric embryo generation via blastocyst microinjection and morula aggregation.
- Embryo transfer is crucial for developing these embryos to term in a surrogate mother.
Purpose of the Study:
- To describe the surgical techniques for reimplanting chimeric embryos into pseudopregnant recipient mice.
- To detail methods for generating pseudopregnant females for embryo transfer.
Main Methods:
- Detailed description of four surgical techniques for embryo reimplantation.
- Explanation of embryo transfer at blastocyst, oocyte, or two-cell stages.
- Methods for creating pseudopregnant females through mating with sterile males, including vasectomy techniques.
Main Results:
- Successful reimplantation of chimeric embryos into pseudopregnant females enables development to term.
- Established protocols for embryo transfer and pseudopregnancy induction.
Conclusions:
- Effective embryo transfer techniques are essential for advancing chimeric embryo research.
- The described methods provide a comprehensive guide for reproductive surgical procedures in mice.
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