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Updated: Jun 22, 2026

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus
Published on: December 22, 2023
Sequence, expression divergence, and complementation of homologous ALCATRAZ loci in Brassica napus
Shuijin Hua1, Imran Haider Shamsi, Yuan Guo
1Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, 268 Kaixuan Road, 310029, Hangzhou, People's Republic of China.
Abstract:
The genomic era provides new perspectives in understanding polyploidy evolution, mostly on the genome-wide scale. In this paper, we show the sequence and expression divergence between the homologous ALCATRAZ (ALC) loci in Brassica napus, responsible for silique dehiscence. We cloned two homologous ALC loci, namely BnaC.ALC.a and BnaA.ALC.a in B. napus. Driven by the 35S promoter, both the loci complemented to the alc mutation of Arabidopsis thaliana, yet only the expression of BnaC.ALC.a was detectable in the siliques of B. napus. Sequence alignment indicated that BnaC.ALC.a and BolC.ALC.a, or BnaA.ALC.a and BraA.ALC.a, possess a high level of similarity. The understanding of the sequence and expression divergence among homologous loci of a gene is of due importance for an effective gene manipulation and TILLING (or ECOTILLING) analysis for the allelic DNA variation at a given locus.
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