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Updated: Jun 22, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
The efficacy of a double-layer collagen membrane technique for overlaying block grafts in a rabbit calvarium model
Su-Hwan Kim1, Do-Young Kim, Kyoung-Hwa Kim
1Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
Objectives:
To analyze the outcomes of a single- and a double-layered collagen membrane on the efficacy of onlay block grafts in terms of bone resorption and augmentation.
Material And Methods:
A total of 36 New Zealand white rabbits were used in this study. Calvarial bone blocks were obtained from one side of the parietal bone and fixed on the contaralateral side. The onlay grafts were covered with either no (C group), one (M1 group), or two (M2 group) layers of a non-cross-linked collagen membrane (BioGide((R))). After 2, 4, and 6 months of healing, rabbits were sacrificed and explanted specimens were prepared for histologic and histomorphometric analysis. At each period, the overall pattern of graft bone resorption and membrane biodegradation were examined histologically, and the sustained external form of grafted bone (%) and the remaining mineralized bone volume (%) were measured histomorphometrically.
Results:
The M1 and M2 groups exhibited decreased bone resorption compared with the C group at all periods. The M2 group had less graft resorption and a higher bone density of the grafted bone than the M1 group. In the M1 group, the collagen membrane was degraded partially or completely by 4 months and was absent at 6 months. Conversely, for the M2 group, the membrane body was retained up to 6 months.
Conclusions:
Our results demonstrated that the collagen membrane used here can reduce graft bone resorption. Furthermore, the double-layer technique using non-cross-linked collagen membranes (BioGide((R))) can enhance the efficacy of the onlay block bone graft technique in terms of both bone resorption and augmentation compared with a single-layer collagen membrane.
