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Updated: Jun 22, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

TOE1 interacts with p53 to modulate its transactivation potential.

Sabina Sperandio1, Saverio Tardito, Aleksandra Surzycki

  • 1Centre de Recherche du CHUL, Department of Molecular Endocrinology and Oncology, Laval University, Quebec, QC Canada.

FEBS Letters
|June 11, 2009
PubMed
Summary
This summary is machine-generated.

The TOE1 gene interacts with the p53 tumor suppressor protein, enhancing its transcriptional activity. This suggests TOE1 acts as a co-regulator for p53, impacting cell growth and the cell cycle.

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The TOE1 gene, a target of the Egr1 transcription factor, is involved in regulating cell growth.
  • Egr1 influences cell cycle progression by upregulating p21, causing a delay at the G2/M phase.

Purpose of the Study:

  • To investigate the interaction between the TOE1 gene and the p53 tumor suppressor protein.
  • To characterize the functional consequences of the TOE1-p53 interaction on p53 transcriptional activity.

Main Methods:

  • Investigated the binding of TOE1 to p53, focusing on the C-terminal tetramerization domain.
  • Determined binding kinetics using nanoporous optical interferometry.

Main Results:

  • Confirmed that TOE1 binds to the p53 protein, specifically its tetramerization domain.
  • Demonstrated that this interaction enhances the transcriptional activity of p53-dependent genes.

Conclusions:

  • TOE1 functions as a co-regulator of p53.
  • The interaction between TOE1 and p53 plays a mechanistic role in regulating gene transcription and potentially cell cycle control.