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Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Hepatitis B virus genetic typing using mass-spectrometry
M V Malakhova1, E N Ilina, V M Govorun
1Scientific Research Institute of Physical-Chemical Medicine, Moscow State Medical-Stomatological University, Moscow, Russia. maja_m@mail.ru
Bulletin of Experimental Biology and Medicine
|June 11, 2009
Summary
Hepatitis B virus (HBV) genetic typing in Russian patients revealed genotype D prevalence (83.3%). Mini-sequencing identified genotypes A, C, and mixed infections, offering advantages over direct sequencing for detecting HBV variants.
Area of Science:
- Hepatology
- Virology
- Genetics
Background:
- Hepatitis B virus (HBV) infection is a global health concern.
- Accurate HBV genetic typing is crucial for understanding disease progression and treatment strategies.
- Previous HBV genotype prevalence data in the Russian population is limited.
Purpose of the Study:
- To determine the prevalence of different hepatitis B virus genotypes in the Russian population.
- To evaluate the efficacy of mini-sequencing with MALDI-ToF mass spectrometry for HBV genetic typing.
- To identify HBV genotypes associated with chronic hepatitis B, combined etiology hepatitis, and hepatic cirrhosis.
Main Methods:
- Mini-sequencing coupled with MALDI-ToF mass spectrometry was utilized for HBV genetic analysis.
- The study included HBsAg-positive patients diagnosed with chronic hepatitis B, hepatitis of combined etiology, and hepatic cirrhosis.
- Genotyping was performed on samples from the Russian population.
Main Results:
- Hepatitis B virus genotype D was the most prevalent (83.3%) across all patient groups.
- Genotypes A (5.9%), C (3.6%), and mixed infections (D and C, 7.2%) were identified in patients with chronic hepatitis B and hepatic cirrhosis.
- Chronic hepatitis of combined etiology was exclusively associated with genotype D.
Conclusions:
- Mini-sequencing with MALDI-ToF mass spectrometry is an effective method for HBV genetic typing, capable of detecting mixed infections.
- Genotype D is the predominant HBV genotype in the studied Russian population.
- Further research is needed to elucidate the specific roles of different HBV genotypes in various liver diseases.
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