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Updated: Jun 22, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

GPCR microspot assays on solid substrates.

Ye Fang¹, Joydeep Lahiri

¹Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 11, 2009

Summary

This summary is machine-generated.

Researchers developed air-stable G protein-coupled receptor (GPCR) microarrays for drug screening. These microarrays offer high fidelity for selectivity profiling, addressing challenges in GPCR membrane immobilization and stability.

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Area of Science:

Biochemistry
Biotechnology
Drug Discovery

Background:

Microarrays are valuable for biomolecular analysis due to multiplexing and miniaturization.
Large-scale microarray implementation requires shelf life, mechanical stability, and microplate format compatibility.
G protein-coupled receptor (GPCR) microarrays face challenges with co-immobilizing receptors and lipid membranes, and maintaining GPCR function on surfaces.

Purpose of the Study:

To describe the fabrication of air-stable GPCR microarrays.
To demonstrate the utility of these microarrays for drug selectivity profiling.

Main Methods:

Development of a novel method for creating air-stable GPCR microarrays.
Co-immobilization of GPCRs and associated lipid membranes onto a solid support.
Utilizing the microarrays for high-fidelity drug selectivity profiling.

Main Results:

Successfully fabricated air-stable GPCR microarrays.
Demonstrated high fidelity in selectivity profiling of GPCR drugs.
Overcame challenges related to membrane desorption and surface requirements for GPCR function.

Conclusions:

Air-stable GPCR microarrays are a viable technology for drug discovery.
This approach enhances the practical implementation of GPCR microarray technology for screening.
High-fidelity drug selectivity profiling is achievable with this advanced microarray format.