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In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
Published on: February 23, 2019
Chronic arsenic exposure impairs macrophage functions in the exposed individuals
Nilanjana Banerjee1, Saptarshi Banerjee, Rupashree Sen
1Molecular and Human Genetics Division, Indian Institute of Chemical Biology, Jadavpur, Kolkata 700032, India.
Introduction:
Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals.
Methods:
Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte-monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway.
Results:
In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p < 0.001) loss of cell adhesion capacity, decrease in nitric oxide production, impaired phagocytic capacity, and decreased CD 54 and F-actin expression. Additionally, chronic arsenic exposure affected Rho A-ROCK pathway which in turn impaired macrophage functions.
Discussion And Conclusion:
These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals.
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