Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells
Thomas G Koch1, Preben D Thomsen, Dean H Betts
1Department of Biomedical Sciences, University of Guelph, Ontario, Guelph, Canada. tkoch@uoguelph.ca
Background Aims:
A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs.
Methods:
Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies.
Results And Conclusions:
Our results indicate that PrepaCyte-EQ medium is superior to Ficoll-Paque PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency.
