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Multiplexed microbead immunoassays by flow cytometry for molecular profiling: Basic concepts and proteomics
V V Krishhan1, Imran H Khan, Paul A Luciw
1Department of Chemistry, California State University, Fresno, CA 93740, USA. vvkrishnan@ucdavis.edu
Critical Reviews in Biotechnology
|June 12, 2009
Summary
Flow cytometry, a cell analysis technique, now enables multiplex analysis of soluble analytes using microbead technology. This advancement offers rapid, sensitive detection for diagnostics and therapeutics development.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunology
Background:
- Flow cytometry evolved from automated cell analysis to user-friendly instruments.
- Advances in electronics, lasers, and microfluidics expanded its capabilities.
- Multiplex analysis is now feasible for soluble analytes using labeled microbeads.
Purpose of the Study:
- To review the principles and evolution of flow cytometry.
- To introduce multiplex microbead technology for flow cytometry.
- To discuss applications, advantages, and future directions in translational medicine.
Main Methods:
- Review of scientific literature on flow cytometry principles and applications.
- Description of multiplex microbead technology for analyte detection.
- Comparative analysis of flow cytometry with other detection methods.
Main Results:
- Flow cytometry is a rapid, high-throughput, sensitive, and reproducible technology.
- Multiplex analysis enables simultaneous detection of multiple targets in a single sample.
- Applications span diagnostics, therapeutics development, and proteomics research.
Conclusions:
- Flow cytometry with multiplex microbead technology is crucial for modern biomedical research and diagnostics.
- This technology enhances the detection of proteins and nucleic acids for disease biomarker identification.
- Future directions include large-scale proteomics and advanced translational medicine applications.
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