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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Upsampling

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Related Experiment Video

Updated: Jun 22, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

Superresolution using gray level coding.

Zeev Zalevsky, Pascuala García-Martínez, Javier García

    Optics Express
    |June 12, 2009
    PubMed
    Summary

    This study introduces a novel super-resolution imaging technique using gray level coding. This method enhances spatial resolution without sacrificing temporal or spectral data, relying instead on intensity dynamic range.

    Area of Science:

    • Optics and Imaging Science
    • Digital Image Processing

    Background:

    • Optical and detector limitations restrict imaging resolution.
    • Existing super-resolution methods often require time or wavelength multiplexing.

    Purpose of the Study:

    • To present a super-resolution imaging approach utilizing gray level coding.
    • To overcome inherent resolution limits imposed by optical systems and detectors.

    Main Methods:

    • Employing gray level coding to encode image information.
    • Imaging objects with a limited number of gray levels.
    • Decoding high-frequency spatial features using a gray level lookup table.

    Main Results:

    • Achieved super-resolution by overcoming optical and detector resolution limits.

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    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

    Published on: January 6, 2026

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    Last Updated: Jun 22, 2026

    Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
    13:11

    Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

    Published on: February 10, 2023

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
    06:25

    Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

    Published on: February 12, 2014

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
    07:12

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

    Published on: January 6, 2026

  • The method trades intensity dynamic range for enhanced spatial resolution.
  • Successfully decoded high-frequency features post-imaging.

    • Conclusions:

    • Gray level coding offers an effective super-resolution strategy.
    • This technique provides an alternative to time or wavelength multiplexing for super-resolution.
    • The approach is suitable for objects with limited gray levels.