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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

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Related Experiment Video

Updated: Jun 22, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Alternative splicing and tumor progression.

Claudia Ghigna¹, Cristina Valacca, Giuseppe Biamonti

¹Istituto di Genetica Molecolare - Consiglio Nazionale delle Ricerche, Via Abbiategrasso 207. 27100 Pavia, Italy.

Current Genomics

|June 12, 2009

Summary

This summary is machine-generated.

Alternative splicing dysregulation is common in cancer, impacting gene function and tumor progression. Understanding these splicing changes offers new diagnostic and therapeutic strategies for cancer.

Keywords:

Alternative splicing EMT biomarkers.cancer splicing correction splicing factors

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Last Updated: Jun 22, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Published on: April 26, 2017

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Area of Science:

Molecular Biology
Genetics
Cancer Research

Background:

Alternative splicing significantly expands proteome diversity in eukaryotes.
Aberrant alternative splicing is implicated in numerous human diseases, notably cancer.
Splicing alterations in cancer arise from genetic mutations and changes in regulatory factors.

Purpose of the Study:

To investigate the role of aberrant splicing in cancer development and progression.
To understand the interplay between splicing, transcription, and signaling pathways in cancer.
To explore cancer-associated splicing variants as diagnostic markers and therapeutic targets.

Main Methods:

Analysis of cis-acting splicing elements and splicing regulatory factors in cancer-associated genes.
Examination of splicing profiles in various cancer types.
Investigating the functional impact of specific splicing isoforms on tumor progression.

Main Results:

Mutations and altered regulatory factor activity significantly impact cancer gene splicing.
Specific splicing profiles are associated with distinct cancer types, suggesting isoform-specific roles.
Aberrant splicing is linked to malignant transformation and tumor progression.

Conclusions:

Deciphering aberrant splicing mechanisms is crucial for understanding cancer biology.
Cancer-associated splicing variants hold potential for novel cancer diagnostics and therapeutics.
Targeting splicing correction offers a promising avenue for innovative cancer treatments.