Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SHARPIN stabilizes estrogen receptor α and promotes breast cancer cell proliferation.

Oncotarget·2017

Same author

Curaxin CBL0100 Blocks HIV-1 Replication and Reactivation through Inhibition of Viral Transcriptional Elongation.

Frontiers in microbiology·2017

Same author

[Determination of strobilurin fungicides in fruits and their mass fragmentation routes by ultra performance liquid chromatography-tandem mass spectrometry].

Se pu = Chinese journal of chromatography·2017

Same author

Effect of mono-(2-ethylhexyl) phthalate (MEHP) on proliferation of and steroid hormone synthesis in rat ovarian granulosa cells in vitro.

Journal of cellular physiology·2017

Same author

Clinical, psychological features and quality of life of fibromyalgia patients: a cross-sectional study of Chinese sample.

Clinical rheumatology·2017

Same author

Escin induces caspase-dependent apoptosis and autophagy through the ROS/p38 MAPK signalling pathway in human osteosarcoma cells in vitro and in vivo.

Cell death & disease·2017

Search research articles

Related Experiment Video

Updated: May 22, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Innovative Applications and Challenges of Isothermal Amplification Technology in miRNA Detection.

Xuebo Li¹, Zhonglin Zhang², Jian Zhu²

¹Department of Laboratory Medicine, Clinical Laboratory Medicine Research Center, West China Hospital, Sichuan University, Sichuan Clinical Research Center for Laboratory Medicine, Chengdu, 610000, PR China.

Current Genomics

|May 21, 2026

Summary

MicroRNAs (miRNAs) are vital biomarkers for cancer diagnosis. Isothermal amplification methods offer a faster, simpler alternative to traditional techniques for point-of-care miRNA detection.

Keywords:

CRISPR/Cas isothermal amplification miRNA nanobiosensing tumor biomarkers

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: May 22, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

MicroRNAs (miRNAs) are endogenous non-coding RNAs (18-25 nucleotides) crucial for biological processes and cancer diagnostics.
Traditional miRNA detection methods (qPCR, Northern blot, microarrays) are sensitive but require complex equipment and time, hindering clinical point-of-care applications.

Purpose of the Study:

To systematically review isothermal amplification technologies for miRNA detection.
To explore advancements and innovation patterns in these technologies.
To support the development of miRNA detection platforms for clinical point-of-care testing.

Main Methods:

Review of isothermal amplification techniques including LAMP, RCA, SDA, and EXPAR.
Analysis of their principles, recent progress, and integration strategies.
Focus on advantages like speed, sensitivity, and operational simplicity.

Main Results:

Isothermal amplification offers sensitive and rapid miRNA detection without thermal cycling.
These methods overcome limitations of traditional techniques for point-of-care applications.
Various isothermal amplification strategies show promise for clinical miRNA diagnostics.

Conclusions:

Isothermal amplification technologies represent a significant advancement in miRNA detection.
These methods are well-suited for developing user-friendly, point-of-care diagnostic tools.
Further development can enhance clinical utility for early cancer diagnosis and monitoring.