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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Updated: Jun 22, 2026

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Surface-supported gold cages.

R Ferrando¹, G Barcaro, A Fortunelli

¹Dipartimento di Fisica and CNISM, Via Dodecaneso 33, Genova, I16146, Italy.

Physical Review Letters

|June 13, 2009

Summary

This summary is machine-generated.

Gold clusters on MgO surfaces form unique cage structures, unlike those found in gas phases. These novel open pyramidal hollow cages are driven by specific gold-gold and gold-magnesium oxide interactions.

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Area of Science:

Materials Science
Surface Science
Computational Chemistry

Background:

Understanding the structural properties of metal clusters on oxide surfaces is crucial for catalysis and nanomaterials.
Gold clusters exhibit unique behaviors when supported on surfaces compared to their gas-phase counterparts.

Purpose of the Study:

To investigate the preferred structures of gold clusters on the MgO(001) surface.
To identify novel structural motifs and the underlying interaction mechanisms.

Main Methods:

Density-functional theory (DFT) calculations were employed to explore the potential energy surface.
Analysis of atomic interactions, including many-body and directional effects, was performed.

Main Results:

Gold clusters in the size range of 23-42 atoms preferentially adopt cage structures on MgO(001).
A new family of structures, termed 'open pyramidal hollow cages,' was discovered, distinct from gas-phase clusters.
The formation of these cages is attributed to specific Au-Au and Au-MgO interactions, including strong many-body and directional effects.

Conclusions:

The MgO(001) surface stabilizes unique cage structures for gold clusters, expanding the known structural families of metal clusters.
The findings highlight the significant role of surface interactions in dictating cluster morphology and stability.