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Updated: Jun 22, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Stability of a surface-bound oligonucleotide duplex inferred from molecular dynamics: a study of single nucleotide
Thomas Naiser1, Jona Kayser, Timo Mai
1Experimentalphysik I, Universität Bayreuth, D-95440 Bayreuth, Germany.
Abstract:
Microarray technology uses the sequence dependent hybridization (binding) affinity of surface-bound oligonucleotide strands for the quantification of complex nucleic acid mixtures. In spite of its huge potential in life science and medicine, microarray oligonucleotide hybridization remains far from being understood. Taking advantage of microarray combinatorial possibilities we show that, although surface bound, the hybridization affinities of single-base mismatched oligonucleotides can be derived from first principles using parameters from bulk.
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